In the dairy industry, cleaning and disinfection of surfaces are important issues and development of innovative strategies may improve food safety. This study was aimed to optimize the combined effect of alkaline electrolyzed water (AEW) and neutral electrolyzed water (NEW) as s were significantly affected by surface roughness electropolished SSP required 10 min, 100 mg/L AEW at 30 C, whereas SSP without modification required 30 min, 300 mg/L AEW at 30 C. From confirmatory tests cells removed were 3.90 0.25 log CFU/cm2 for electropolished SSP, and 3.20 0.20 log CFU/cm2 for SSP without modification. NEW is non-corrosive, and can be advantageously used for environmentally friendly cleaning and disinfection processes.

Application of slightly acidic electrolyzed water (SAEW) in combination with ultrasound for decontamination of kashk was investigated. SAEW had a pH of 5.3-5.5, an oxidation reduction potential of 545-600 mV, and an available chlorine concentration of 20-22 mg/L. Kashk is a dairy product with a unique aroma and a high nutritive value produced in Iran. A 2/1 SAEW/kashk ratio showed 1.42, 1.13, 1.24, and 1.37 log CFU/mL microbial reductions in Staphylococcus aureus, Bacillus cereus, Escherichia coli, and Aspergillus fumigatus, respectively, at room temperature. A combination of SAEW treatment with ultrasound (SAEWultrasound) resulted in 1.87, 1.67, 1.71, and 1.91 log CFU/mL reductions in S. aureus, B. cereus, E. coli, and A. fumigatus, respectively. The developed hurdle approach can be a useful tool for sanitization of kashk and similar products. Application of SAEWultrasound in dairy microbial decontamination is first reported herein.

Electrolysed oxidizing (EO) water is produced by passing a DC current through a weak sodium chloride solution, resulting in alkaline and acidic EO water. The goal of this research was to determine whether EO water could be used as an acceptable cleaning and disinfecting agent for materials used in pipeline milking systems. Small pieces of materials commonly used in milking systems were soiled using raw milk inoculated with a cocktail of four bacterial cultures similar to those commonly found in raw milk, and then cleaned by soaking in alkaline EO water followed by soaking in acidic EO water at various treatment times and temperatures. Effective treatment time and temperature combinations were determined by response surface design for a temperature range of 2560C and a time range of 520 min. Treated materials were evaluated by aerobic plate counts and ATP bioluminescence to assess the disinfecting and cleaning effectiveness of EO water. Most of the treatments at 60C and several treatments at lower temperatures successfully removed all detectable bacteria and ATP. Overall, the results of this study indicated that EO water has the potential to be used as a cleaning and disinfecting agent for materials used in milking systems.

Milking systems, electrolyzed oxidizing water, CIP, clean in place, ATP bioluminescence Electrolyzed oxidizing (EO) water is a technology that electrolyzes a weak sodium chloride solution into sodium and chlorine, resulting in two solutions alkaline and acid. The goal of this research is to determine if EO water is an acceptable cleaning agent for pipeline milking systems. After constructing 1.5 inch-diameter pipeline milking system, the system was soiled using raw milk that had been spiked with common raw milk microorganisms. After soiling, the system was rinsed with warm water and then evaluated in several locations for initial counts. To evaluate the level of soiling, the surfaces were evaluated using an ATP bioluminescence method. The milk contact surfaces were also swabbed for microbial analysis. The pipeline system was then washed with an alkaline EO water treatment followed by an acidic EO water treatment. After treatment, the effectiveness of the treatment was evaluated by ATP bioluminescence and microbiological analysis. First, a 10 min wash with 60C alkaline water followed by a 10 min wash with 60C acid water successfully removed all detectable bacteria and ATP from the non-porous milk contact surfaces. Shorter treatment times (5 and 7.5 min) with EO water were also tested, along with a control treatment using conventional dairy cleaning chemicals. Using ANOVA, there were no significant differences between the EO water treatments and the conventional treatment, however the 5-min EO water treatment was significantly less effective than the 10-min treatment.